• Costa B, Li Calzi M, Castellano M, Blanco V, Cuevasanta E, Litvan I, Ivanov P, Witwer K, Cayota A, Tosar J.P.* (2023). Nicked tRNAs are stable reservoirs of tRNA halves in cells and biofluids. P.N.A.S., accepted, in press.  120(4) e2216330120. DOI. PubMed

Comment in: Staying together after the breakup: tRNA halves in extracellular fluids

• Tosar JP*, Cayota A, Witwer K (2022). Exomeres and Supermeres: monolithic or diverse? Journal of Extracellular Biology. DOI. PubMed

• Mateescu B, Jones J, Alexander R, et al. (2022). Phase 2 of extracellular RNA communication consortium charts next-generation approaches for extracellular RNA research. iScience. DOI. PubMed

• Marquez ME, Sernbo S, Payque E, Uria R, Tosar JP, Querol J, Berca C, Uriepero A, Prieto D, Álvarez D,  Oliver C, Irigoin V, Dos Santos G, Guillermo C, Landoni AI, Navarrete M, Palacios F, Oppezzo P (2022). TGF-β/SMAD Pathway Is Modulated by miR-26b-5p: Another Piece in the Puzzle of Chronic Lymphocytic Leukemia Progression. Cancers 14 (7), 1676. DOI. 

• Tosar JP* (2022). Costo de los cargos por procesamiento de artículo (APC) para Uruguay: el precio desmedido del acceso abierto. Informatio 27 (1), 221-252. Scielo.org.

• Alexander RP, Kitchen RR, Tosar JP, Roth M, Mestdagh P, Max KEA, Rozowsky J, Kaczor-Urbanowicz KE, Chang J, Balaj L, Losic B, Van Nostrand EL, LaPlante E, Mateescu B, White BS, Yu R, Milosavljevic A, Stolovitzky G, Spengler RM. (2022) Open Problems in Extracellular RNA Data Analysis: Insights From an ERCC Online Workshop. Frontiers in Genetics 12:778416. DOI. PubMed.

• Fagúndez P, Botasini S, Tosar JP, Méndez E (2021) Systematic process evaluation of the conjugation of proteins to gold nanoparticles. Heliyon 7 (6): e07392. DOI. PubMed.

• Tosar JP, Ivanov P, Ribas de Pouplana L, Torres AG (2021) Editorial: Understanding the Importance of Non-Canonical tRNA Function. Frontiers in Molecular Biosciences 8: 769784. DOI. PubMed.

• Tosar JP* (2021) Die hard: resilient RNAs in the blood. Nature Reviews Molecular Cell Biology 22(6):373.  DOI. PubMed

• Tosar JP*, Gámbaro F, Castellano M, Cayota A (2021) RI-SEC-seq: Comprehensive Profiling of Nonvesicular Extracellular RNAs with Different Stabilities. Bio-protocol 11(4): e3918. DOI. PubMed

• Arab T,  Mallick E, Huang Y, Dong L, Liao Z, Zhao Z, Gololobova O, Smith B, Haughey N, Pienta K, Slusher B, Tarwater P, Tosar JP, Zivkovic A, Vreeland W, Paulaitis M, Witwer KW (2021) Characterization of extracellular vesicles and synthetic nanoparticles with four orthogonal single-particle analysis platforms. Journal of Extracellular Vesicles 10(6): e12079. DOI. PubMed

• Tosar JP*, Witwer KW, Cayota A (2021) Revisiting Extracellular RNA Release, Processing, and Function. Trends in Biochemical Sciences 46(6): 438-445. DOI. PubMed

• Tosar JP*, García-Silva MR, Cayota A (2021) Circulating SNORD57 rather than piR-54265 is a promising biomarker for colorectal cancer: common pitfalls in the study of somatic piRNAs in cancer. RNA 27(4): 403-410. DOI. PubMed 

• Tosar JP*, Segovia M, Castellano M, Gámbaro F, Akiyama Y, Fagúndez P, Olivera A, Costa B, Possi T, Hill M, Ivanov P, Cayota A* (2020) Fragmentation of extracellular ribosomes and tRNAs shapes the extracellular RNAome. Nucleic Acids Research 48(22): 12874-12888, DOI. PubMed 

• Tosar JP*, Cayota A* (2020) Extracellular tRNAs and tRNA-derived fragments. RNA Biology 17(8): 1149 - 1167. DOI. PubMed

• Fagúndez P, Brañas G, Laíz J, Tosar JP.* (2020) Electrochemical Detection of dsDNA-Specific Antibodies. Methods in Molecular Biology 2063: 73-83, DOI. PubMed

• Gámbaro F, Li Calzi M, Fagúndez P, Costa B, Greif G, Mallick E, Lyons S, Ivanov P, Witwer K, Cayota A*, Tosar JP.* (2020) Stable tRNA halves can be sorted into extracellular vesicles and delivered to recipient cells in a concentration-dependent manner. RNA Biology 17(8): 1168 - 1182.  DOI. PubMed

• Fernández-Calero T, Davyt M, Perelmuter K, Chalar C, Bampi G, Persson H, Tosar JP, Hafstao V, Naya H, Rovira C, Bollati-Fogolin M, Ehrlich R, Flouriot G, Ignatova Z, Marín M (2020) Fine-tuning the metabolic rewiring and adaptation of translational machinery during an epithelial-mesenchymal transition in breast cancer cells. Cancer & Metabolism 8, 8. DOI. PubMed. 

• Fromm B, Tosar JP, Aguilera F, Friedländer MR, Bachmann L, Hejnol A (2019) Evolutionary Implications of the microRNA- and piRNA Complement of Lepidodermella squamata (Gastrotricha). Non-Coding RNA  5, e19. DOI. PubMed 

• Fromm B, Kang W, Rovira C, Cayota A, Witwer KW*, Friedländer M*, Tosar JP* (2019) Plant microRNAs in human sera are likely contaminants. Journal of Nutritional Biochemistry 65: 139-140. DOI. PubMed

• Théry C, Witwer K, et al. (2018) Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines. Journal of Extracellular Vesicles 7 (1), e1535750. DOI. PubMed

• Tosar JP,*; Gámbaro F, Darré L, Pantano S, Westhof E, Cayota A* (2018) Dimerization confers increased stability to nucleases in extracellular 5’ halves from glycine and glutamic acid tRNAs. Nucleic Acids Research 46 (17), 9081-9093. DOI. PubMed

• Fromm B, Tosar JP, Yu L, Halushka M, Witwer K (2018). Human and Cow Have Identical miR-21-5p and miR-30a-5p Sequences, Which Are Likely Unsuited to Study Dietary Uptake from Cow Milk. Journal of Nutrition, 148 (9), 1506-1507. DOI. PubMed

• Tosar JP, Cayota A (2018) Detection and analysis of non-vesicular extracellular RNA. Methods in Molecular Biology 1740: 125-137. DOI. PubMed

• Fagúndez P, Brañas G, Cairoli E, Laíz J, Tosar JP* (2018). An electrochemical biosensor for rapid detection of anti-dsDNA antibodies in absolute scale. Analyst  143:3874-3882. DOI. PubMed

• Tosar JP*, Rovira C, Cayota A (2018) Non-coding RNA fragments account for the majority of annotated piRNAs expressed in somatic non-gonadal tissues.  Communications Biology 1, e2. DOI. PubMed

• Tosar JP, Cayota A, Eitan E, Halushka MK, Witwer KW (2017) Ribonucleic artefacts: are some extracellular RNA discoveries driven by cell culture medium components? Journal of Extracellular Vesicles 6 (1), e1272832. DOI. PubMed

• Mateescu B, Kowal EJK, van Balkom BWM, et al. (2017) Obstacles and opportunities in the functional analysis of extracellular vesicle RNA–an ISEV position paper. Journal of Extracellular Vesicles 6 (1), e1286095. DOI. PubMed 

•  Doldán X, Fagúndez P, Cayota A, Laíz J, Tosar JP* (2016) Electrochemical sandwich immunosensor for determination of exosomes based on surface marker-mediated signal amplification. Analytical Chemistry 88 (21): 10466-10473. DOI. PubMed

• Tosar JP, Gámbaro F, Sanguinetti J, Bonilla B, Witwer KW, Cayota A (2015) Assessment of small RNA sorting into different extracellular fractions revealed by high-throughput sequencing of breast cell lines. Nucleic Acids Research 43 (11): 5601-5616. DOI. PubMed

• Tosar, J.P.; Rovira, C.; Naya, H.; Cayota, A. (2014) Mining of public sequencing datasets supports a non-dietary origin for putative foreign miRNAs: underestimated effects of contamination in NGS. RNA 20 (6): 754-757. DOI. PubMed

• Tosar JP, Holmes J, Collyer S, Davis F, Laíz J,* Higson H (2013) Template and catalytic effects of DNA in the construction of polypyrrole/DNA composite macro and microelectrodes. Biosensors & Bioelectronics, 41: 294-301.  DOI. PubMed

• García-Silva M.R.1; Tosar, J.P1.; Frugier, M.; Pantano, S.; Bonilla, B.; Esteban, L.; Serra, E.; Rovira, C.; Robello, C.; Cayota, A. (2010) Cloning, characterization and subcellular localization of a trypanosoma cruzi argonaute protein defining a new subfamily distinctive of trypanosomatids . Gene 466: 26 - 35. DOI. PubMed

• García-Silva M.R.; Frugier, M.; Tosar, J.P.; Correa Dominguez, A.; Ronalte Alvez, L.; Parodi-Talice, A.; Rovira, C.; Robello, C.; Goldenberg, S.; Cayota, A (2010) A population of tRNA-derived small RNAs is actively produced in Trypanosoma cruzi and recruited to cytoplasmic granules. Molecular and Biochemical Parasitology 171 (2): 64 – 73. DOI. PubMed

• Tosar JP, Brañas G, Laíz J* (2010). Electrochemical DNA hybridization sensors applied to real and complex biological samples. Biosensors & Bioelectronics 26 (4): 1205-1217. DOI. PubMed

• Tosar JP, Keel K, Laíz J* (2009) Two independent label-free detection methods in one electrochemical DNA sensor. Biosensors & Bioelectronics 24 (10): 3036 – 3042. DOI. PubMed

2019 - 2023: Separation methods for exRNA carriers: extracellular vesicles, lipoprotein particles, and protein aggregates. PI: Kenneth Witwer. Role: Key Personnel. NIH Extracellular RNA Communication Consortium. National Institutes of Health, USA. 

2022-2024: Extracellular RNA decoding by innate immune sensors. PI: Juan Pablo Tosar. National Agency for Research and Innovation (ANII), Uruguay.  (FCE_1_2021_1_166344)

2021-2023: Extracellular RNA biogenesis  and role in intercellular communication. PI: Juan Pablo Tosar. CSIC, Universidad de la República, Uruguay.

2019-2021: Release of RNAs from the translation machinery as a stress-response mechanism. PI: Juan Pablo Tosar. National Agency for Research and Innovation (ANII), Uruguay.  (FCE_3_2018_1_148745)

2017-2019: Biosensors for descentralized detection of exosomes and Dengue virus. PI: Juan Pablo Tosar. CSIC, Universidad de la República, Uruguay. 

2013 - 2015: extracellular RNAs in cancer: implications in intercellular communication between malignant and non-malignant cells. PI: Juan Pablo Tosar. National Agency for Research and Innovation (ANII), Uruguay.  

Tosar, J.P: Extracellular stable nonvesicular RNAs. In: 1st Annual Meeting of the exRNA-PATH Consortium (Turin, Italy). June, 2022  Invited oral presentation

Tosar, J.P:  Metabolism of extracellular ribosomes and tRNAs & identification of stable extracellular fragments.  In: Seminars of the Department of Biochemistry and Molecular Biology of the University of Hamburg. June, 2022. Virtual seminar, invited oral presentation. 

Tosar, J.P:  Processing and signaling potential of exRNAs transported inside and outside extracellular vesicles. In: Seminars of the Max Planck Institute for Infection Biology. Berlin (Germany). June, 2022. Invited oral presentation.

Costa, B., Castellano, M., Blanco, V., Li Calzi, M., Cuevasanta, E., Ivanov, P., Witwer, K., Cayota, A., Tosar, J.P.: identification of stable naked RNAs in biofluids. In: RNA 2022, 27th Annual Meeting of the RNA Society. Boulder (USA). June, 2022. Poster presentation.

Tosar, J.P.: Extracellular vesicles and extracellular RNA: from intercellular communication to biomarker discovery. In: Congress of the Argentinian Society for Nanomedicine (NANOMED-AR). Keynote, invited (virtual).

Tosar, J.P, et. al.:  Fragmentation of extracellular ribosomes and tRNAs shapes the extracellular RNAome. In: ISEV 2020 Annual Meeting (abstract selected for oral presentation).  

Tosar, J.P: Fragmentation of extracellular ribosomes and tRNAs shapes the extracellular RNAome. In: LV annual meeting of the Sociedad Argentina de Investigación en Bioquímica (SAIB), joint PABMB meeting. Salta (Argentina), November, 2020. Invited oral presentation.

Tosar, J.P: Glycine tRNA Halves Are Intrinsically Stable Entities Which Can Be Secreted Non-Selectively in EVs and Incorporated in Recipient Cells in a Homotypic Human Cell Model. In: Gordon Research Conference in Extracellular Vesicles. Newry, Maine (USA). August, 2018. Oral presentation, selected.

Tosar, J.P.: Estabilidad, liberación y reconocimiento de RNAs extracelulares no asociados a vesículas. In: Workshop Biología Celular y Molecular del ARN. Instituto Leloir. Buenos Aires (Argentina). April, 2018. Invited oral presentation.

Tosar, J.P, Gámbaro, F.; Segovia, M.; Hill, M.; Westhof, E.; Cayota, A.: Extracellular-enriched tRNA halves assemble into nuclease-resistant dimers and high molecular weight aggregates with potent immunostimulatory activity. In: Gordon Research Conference in Nucleic Acids. Maine (USA). June, 2017. Oral presentation in the associated Gordon Research Seminar (GRS). Poster presentation in the GRC.

Tosar, J.P, Gámbaro, F.; Segovia, M.; Hill, M.; Westhof, E.; Cayota, A.: Extracellular-enriched tRNA halves assemble into nuclease-resistant dimers and high molecular weight aggregates with potent immunostimulatory activity. In: 22nd Annual Meeting of the RNA Society. Prague (Czech Republic) May, 2017. Poster presentation.

Tosar, J.P, Cayota, A.: Comparing intra- and extracellular small RNA profiles to estímate secretion mechanisms (selective vs. non-selective) of different small RNA families into extracellular vesicles. In: EV-associated RNA, what is the purpose? Workshop of the International Society for Extracellular Vesicles. Utrecht (The Netherlands). September, 2015. Oral presentation.

Tosar, J.P.; Sanguinetti, J.; Gambaro, F.; Bonilla, B.; Cayota, A. The small RNA content secreted by benign (MCF-10A) and malignant (MCF-7) mammary epithelial cell lines in different extracellular fractions. In: RNA 2014. 19th annual meeting of the RNA Society. Québec (Canada). June, 2014. Poster presentation.

Tosar, J.P.; Holmes, J.; Collyer, S.; Davis, F.; Laíz, J.; Higson, H. Template and catalytic effects of DNA in the construction of polypyrrole/DNA composite macro and microelectrodes. In: Biosensors 2012. 22th Anniversary World Congress on Biosensors, 2012 Cancún (México). May, 2012. Poster presentation.

Tosar, J.P.; García-Silva M.R.; Serra, E.; Pantano, S.; Bonilla, B.; Robello, C.; Cayota, A. TcPIWI-tryp: a PAZ/PIWI domain-containing protein (Argonaute) from the RNAi negative parasitic protozoan Trypanosoma cruzi. In: 3rd Latin American Protein Society Meeting. Salta (Argentina). October, 2010. Poster presentation.

Tosar, J.P.; García-Silva, M.R.; Serra, E.; Pantano, S.; Bonilla B.; Robello, C.; Cayota, A.; New insights into Argonaute evolution: a different evolutionary origin for trypanosomatid Argonautes? 150 years of Darwin’s evolutionary theory: a South American celebration. Punta del Este (Uruguay) September, 2009. Poster presentation.

Current PhD students:

Pablo Fagúndez. In collaboration with Dr. Eduardo Méndez. 

Mauricio Castellano. In collaboration with Dr. Mercedes Segovia.

Marco Li Calzi. In collaboration with Dr. Alfonso Cayota.

Valentina Blanco. In collaboration with Dr. Alfonso Cayota.

Current MSc students:

Bruno Costa. In collaboration with Dr. Alfonso Cayota.

Former graduate students: 

Marco Li Calzi, 2020.  Currently doing his PhD with us.

Fabiana Gámbaro, 2018. Currently doing her PhD thesis at Institut Pasteur de París, France.

Former undegraduate students (BSc): 

Bruno Costa, 2020.

Fabiana Gámbaro, 2017.

Ximena Doldán, 2016.

RNA Society: member.  Organizer of funded RNA Salons: 2018/2019, 2019/2020, 2020/2021 ("Uruguayan RNA Club").

International Society for Extracellular Vesicles (ISEV): member

Uruguayan RNA Club (Club Uruguayo del ARN): founder member. Current member of the organizing comittee

Sociedad Uruguaya de Bioquímica y Biología Molecular (SBBM): member.

Sociedad Uruguaya de Biociencias (SUB): member.

Journal of Extracellular Vesicles, Nucleic Acids Research, Biochemical Journal, Advanced Science, Carcinogenesis, Cancer Medicine, J. Molecular Medicine, Theranostics, Biochemical Genetics, ACS Biomaterials Science & Engineer, RNA, Gene Therapy, Experimental Gene Therapy, Analytical Chemistry, Analytical Biochemistry, Scientific Reports, Communications Biology, ACS Applied Materials & Interfaces, Current Analytical Chemistry, Peer J, Current Microbiology, Plos Neglected Tropical Diseases, RNA Biology, Frontiers in Molecular Biosciences, F1000 Research, Biosensors and Bioelectronics, J. Experimental Botany, Pharmaceuticals. SEE ALL

I have also reviewed research grants, early career researcher grants and course proposals for: Welcome Trust India Alliance (India), Foncyt (Argentina), UN-Biolac (United Nations University). ANII (Uruguay), CSIC (Uruguay) and MEC (Uruguay),